The Katedrála Sv. Petra a Pavla (The Cathedral of Saint Peter and Paul) represents one of the most notable architectural and religious buildings in the city of Brno. The original holy place was initially built as a chapel in the 11th century, but it gradually transformed over the centuries, in a Baroque-style place of worship. Its main attractions are the two 84 meter-high towers and the magnificent Gothic interior.

The Church of the Holy Cross (Kostel Nalezení Svatého Kříže) is located on the Capuchin Square in downtown Brno. The holy building has been erected by the order of Capuchins in the 17th century in order to meet the needs of a growing number of followers of the order. The church was built in Baroque style and has a simple design. The main attraction of the church is the "Crypto Capuccinorum" (Capuchin crypt).

The Church of Saint Mary Magdalene (Kostel Svaté Máří Magdalény) was built in the 15th century on the site where a Jewish synagogue once stood. The city's records show that the synagogue had been occupied by force by the Franciscans and converted into the present church. The Franciscans have also gave it a new Romanesque touch and an adjacent tower.

St. Johns' Church (Kostel Svatých Janů) is a Roman Catholic cathedral built by the Minorire order in the 13th century in the historical centre of Brno. Over the centuries, the church has undergone some major improvements, from Gothic to Baroque style and also a new chapel (Loreto Chapel) has been added to the construction. The church is popular among the locals due to its traditional baptizing and other interesting traditions.

St. Michael's Church (Kostel Svatého Michala) is yet another church in Brno built by the Dominican Order in the 13th century. It is located on the Dominican square, thus making it very accessible and very visited by locals and tourists. After the swedish attack in 1645, it got a new Baroque appearance with the help of architect Jan Baptist Erna. The service is held regularly and everyone is welcome for daily prayers.

The Assumption of Virgin Mary Church (Kostel Nanebevzetí Panny Marie) is one of the 3 Jesuit churches that the Jesuit order has built in Brno. This church is also associated with the name of the Jesuit college, which played an important part in the history of the city by providing knowledge to the local peasants free of charge. The construction is one of the earliest Baroque buildings in Brno.

The St. James' Church (Kostel Svatého Jakuba) is a late Gothic architectural masterpiece and church in Brno. Its history dates back to the early 13th century. The church is the tomb of the famous defender of Brno - Louise de Raduit Souches. The church's ceiling is known for its numerous guild features, crests of noble families and various monograms. The holy building was declared a national cultural monument in 1995.

The St. Thomas' Church (Kostel Svatého Tomáše) was established by  Jan Jindřich, a Czech prince, and was originally an Augustinian monastery. This holy building is also the burial place of some great sovereigns such as Margrave Jobst. The building has changed its architectural design over the years, and its current Baroque appearance dates from the 17th century.